mouse spleen magcellect mouse nk cell isolation kit Search Results


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Miltenyi Biotec spleen dissociation kit
Spleen Dissociation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TaKaRa nucleospin rna plus kit
Nucleospin Rna Plus Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science mouse splenic lymphocyte isolation kit p8860
Mouse Splenic Lymphocyte Isolation Kit P8860, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc easysep mouse t-cell negative isolation kit
Easysep Mouse T Cell Negative Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc mouse cd11c positive selection kit
Mouse Cd11c Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen dneasy® blood & tissue kit
Dneasy® Blood & Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec macs nk isolation kit
Macs Nk Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec macs cd8 microbead kit
Macs Cd8 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec spleen cd4 cd62l t cells
Analysis of T and B cells in total lung cells of OVA-treated IL-3 −/− mice (A andB) Flow cytometry analysis of CD19 + CD45R + B cells (n = 5) (a) and CD103 + cDCs (n = 4/4/5/5) (b) cells in total lung cells isolated from BALB/c WT and IL3 −/− mice with and without asthma. (C) ELISA analysis of the IL-4 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15). (D and E) <t>CD4</t> + and CD8 + T cells (d) and Foxp3 + CD25 + CD4 + T reg (e) (n = 10 (CD4 + cells), n = 5 (CD8 + , T reg )). A representative dot plot is shown for each group. Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
Spleen Cd4 Cd62l T Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec mouse cd4 t cell isolation kit
Analysis of T and B cells in total lung cells of OVA-treated IL-3 −/− mice (A andB) Flow cytometry analysis of CD19 + CD45R + B cells (n = 5) (a) and CD103 + cDCs (n = 4/4/5/5) (b) cells in total lung cells isolated from BALB/c WT and IL3 −/− mice with and without asthma. (C) ELISA analysis of the IL-4 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15). (D and E) <t>CD4</t> + and CD8 + T cells (d) and Foxp3 + CD25 + CD4 + T reg (e) (n = 10 (CD4 + cells), n = 5 (CD8 + , T reg )). A representative dot plot is shown for each group. Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
Mouse Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Miltenyi Biotec tumor dissociation kit
Analysis of T and B cells in total lung cells of OVA-treated IL-3 −/− mice (A andB) Flow cytometry analysis of CD19 + CD45R + B cells (n = 5) (a) and CD103 + cDCs (n = 4/4/5/5) (b) cells in total lung cells isolated from BALB/c WT and IL3 −/− mice with and without asthma. (C) ELISA analysis of the IL-4 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15). (D and E) <t>CD4</t> + and CD8 + T cells (d) and Foxp3 + CD25 + CD4 + T reg (e) (n = 10 (CD4 + cells), n = 5 (CD8 + , T reg )). A representative dot plot is shown for each group. Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
Tumor Dissociation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd138 isolation kit
Analysis of T and B cells in total lung cells of OVA-treated IL-3 −/− mice (A andB) Flow cytometry analysis of CD19 + CD45R + B cells (n = 5) (a) and CD103 + cDCs (n = 4/4/5/5) (b) cells in total lung cells isolated from BALB/c WT and IL3 −/− mice with and without asthma. (C) ELISA analysis of the IL-4 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15). (D and E) <t>CD4</t> + and CD8 + T cells (d) and Foxp3 + CD25 + CD4 + T reg (e) (n = 10 (CD4 + cells), n = 5 (CD8 + , T reg )). A representative dot plot is shown for each group. Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.
Cd138 Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Analysis of T and B cells in total lung cells of OVA-treated IL-3 −/− mice (A andB) Flow cytometry analysis of CD19 + CD45R + B cells (n = 5) (a) and CD103 + cDCs (n = 4/4/5/5) (b) cells in total lung cells isolated from BALB/c WT and IL3 −/− mice with and without asthma. (C) ELISA analysis of the IL-4 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15). (D and E) CD4 + and CD8 + T cells (d) and Foxp3 + CD25 + CD4 + T reg (e) (n = 10 (CD4 + cells), n = 5 (CD8 + , T reg )). A representative dot plot is shown for each group. Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

Journal: iScience

Article Title: Targeted deletion of Interleukin-3 results in asthma exacerbations

doi: 10.1016/j.isci.2022.104440

Figure Lengend Snippet: Analysis of T and B cells in total lung cells of OVA-treated IL-3 −/− mice (A andB) Flow cytometry analysis of CD19 + CD45R + B cells (n = 5) (a) and CD103 + cDCs (n = 4/4/5/5) (b) cells in total lung cells isolated from BALB/c WT and IL3 −/− mice with and without asthma. (C) ELISA analysis of the IL-4 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15). (D and E) CD4 + and CD8 + T cells (d) and Foxp3 + CD25 + CD4 + T reg (e) (n = 10 (CD4 + cells), n = 5 (CD8 + , T reg )). A representative dot plot is shown for each group. Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

Article Snippet: Spleen CD4 + CD62L + T cells were positively sorted by magnetic bead isolation using the mouse CD4 + CD62L + T cell isolation kit (MACS, Miltenyi Biotec, Bergisch-Gladbach, Germany) in accordance to the manufacturer’s protocol.

Techniques: Flow Cytometry, Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture

Increased T H 2 cytokines in the lung of IL-3 −/− asthmatic mice (A) Flow cytometry analysis of GATA3 + CD4 + T H 2 cells and in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 5). A representative dotplot is shown for each group. (B–D) ELISA analysis of the IL-5, GM-CSF, and IL-13 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15 (IL-5 and IL-13); n = 13/14/14/14 (GM-CSF)). Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

Journal: iScience

Article Title: Targeted deletion of Interleukin-3 results in asthma exacerbations

doi: 10.1016/j.isci.2022.104440

Figure Lengend Snippet: Increased T H 2 cytokines in the lung of IL-3 −/− asthmatic mice (A) Flow cytometry analysis of GATA3 + CD4 + T H 2 cells and in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 5). A representative dotplot is shown for each group. (B–D) ELISA analysis of the IL-5, GM-CSF, and IL-13 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15 (IL-5 and IL-13); n = 13/14/14/14 (GM-CSF)). Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

Article Snippet: Spleen CD4 + CD62L + T cells were positively sorted by magnetic bead isolation using the mouse CD4 + CD62L + T cell isolation kit (MACS, Miltenyi Biotec, Bergisch-Gladbach, Germany) in accordance to the manufacturer’s protocol.

Techniques: Flow Cytometry, Cell Culture, Enzyme-linked Immunosorbent Assay